FAIRMol

Z25065658

Pose ID 644 Compound 415 Pose 644

DB SELECTIONDocking pose analysis is being read from this database.
Molecular metrics status: done
RDKit SASA-based burial metrics are cached.
SASA cached
T01
Human DHFR Human
Ligand Z25065658
PDB5SD8

3D complex viewer

Strict H-bonds Permissive H-bonds
Viewer legend
Protein receptor
Pocket residues
Cofactor context
Docked ligand
Overall assessment
Weak SASA cached
Weak or marginal quality
Binding strong Geometry medium Native strong SASA done
Strain ΔE
21.8 kcal/mol
Protein clashes
2
Internal clashes
3
Native overlap
contact recall 0.71, Jaccard 0.60, H-bond role recall 0.00
Burial
89%
Hydrophobic fit
79%
Reason: no major geometry red flags detected
2 protein-contact clashes 3 intramolecular clashes
Molecular report
Full metrics ↗
Weak Marginal quality. Consider only alongside better-scoring alternatives.
✓ Excellent LE (-0.826 kcal/mol/HA) ✓ Good fit quality (FQ -8.05) ✓ Strong H-bond network (6 bonds) ✓ Deep burial (89% SASA buried) ✓ Lipophilic contacts well-matched (79%) ✗ High strain energy (21.8 kcal/mol) ✗ Geometry warnings ✗ Many internal clashes (10)
Score
-25.606
kcal/mol
LE
-0.826
kcal/mol/HA
Fit Quality
-8.05
FQ (Leeson)
HAC
31
heavy atoms
MW
437
Da
LogP
4.81
cLogP
Strain ΔE
21.8 kcal/mol
SASA buried
89%
Lipo contact
79% BSA apolar/total
SASA unbound
738 Ų
Apolar buried
519 Ų

Interaction summary

HB 6 HY 24 PI 2 CLASH 3
Final rank2.417Score-25.606
Inter norm-0.839Intra norm0.013
Top1000noExcludedno
Contacts19H-bonds6
Artifact reasongeometry warning; 10 clashes; 1 protein clash; 1 cofactor-context clash; moderate strain Δ 21.8
Residues
ALA10 ASP22 GLU31 ILE61 ILE8 LEU23 NAP201 PHE135 PHE180 PHE35 PRO62 SER60 THR137 THR57 TYR122 TYR34 VAL116 VAL136 VAL9

Protein summary

200 residues
Protein targetT01Atoms3128
Residues200Chains2
Residue summaryLYS:374; LEU:361; GLU:240; VAL:224; ARG:192; PHE:180; ILE:171; PRO:168; ASN:140; SER:132; TYR:126; GLN:119; ASP:108; MET:102; THR:98; GLY:91

Receptor context

1 kept / 0 excluded
Receptor context filtering: interactions and SASA are computed against protein atoms plus allowed cofactors/ions. Native ligand-like HETATM partners are excluded from scoring.
Kept context 1 Excluded HETATM 0
Kept cofactors / ions
A:NAP201

Native ligand reference

★ reference
Interaction fingerprint calculated directly from the uploaded native ligand without docking. Current H-bond mode: strict.
Name5SD8Contacts21
PoseOpen native poseHB0
IFP residues
ALA10 ASN65 ASP22 GLN36 GLU31 GLY117 GLY21 ILE61 ILE8 LEU23 LEU68 NAP201 PHE32 PHE35 PRO62 SER60 THR137 THR57 TYR122 VAL116 VAL9
Current overlap15Native recall0.71
Jaccard0.60RMSD-
HB strict0Strict recall0.00
HB same residue+role0HB role recall0.00
HB same residue0HB residue recall0.00

HB · H-bonds

Mode: strict. Count shows atom-level H-bonds; unique residues in summary: 0.

PI · π–π interactions

Native π–π recall is disabled because no explicit native π–π reference was stored.

HY · Hydrophobic contacts

CLASH · Clashes

All stored poses for this docking hit

PoseFinal rankInter normScoreHBCTCT overlapCT recallHB role rec.RMSDExcluded
644 2.416502217508458 -0.838814 -25.6063 6 19 15 0.71 0.00 - no Current

Molecular metrics

RDKit SASA burial, strain energy (MMFF94s), ligand efficiency and fit quality for this docking pose.
✓ Metrics available

Scoring & efficiency

Docking score -25.606kcal/mol
Ligand efficiency (LE) -0.8260kcal/mol/HA
Score / heavy atom count
Fit quality (FQ) -8.047
LE / (0.072 + 0.95/HAC) — Leeson & Springthorpe
Heavy atom count 31HA

Physicochemical properties

Molecular weight 436.5Da
Lipinski: ≤ 500 Da
LogP (cLogP) 4.81
Lipinski: ≤ 5
Rotatable bonds 7

Conformational strain (MMFF94s)

Strain energy (ΔE) 21.84kcal/mol
< 5 good · 5–10 marginal · > 10 problematic
Docked FF energy 76.49kcal/mol
Minimised FF energy 54.65kcal/mol

SASA & burial

✓ computed
SASA (unbound) 738.3Ų
Total solvent-accessible surface area of free ligand
BSA total 658.8Ų
Buried surface area upon binding
BSA apolar 519.2Ų
Hydrophobic contacts buried
BSA polar 139.6Ų
Polar contacts buried
Fraction buried 89.2%
> 60 % indicates good pocket engagement
Lipophilic contact ratio 78.8%
BSA apolar / BSA total — high = hydrophobic driver
Δ Non-polar SASA -1646.9Ų
SASA_nonpolar(complex) − SASA_nonpolar(receptor) − SASA_nonpolar(ligand free). Negative = non-polar surface buried upon binding. Requires full polarity-decomposed SASA computation.
Receptor non-polar SASA 1677.9Ų
Non-polar SASA of receptor alone (VdW proxy, nonpolar atoms only)
Complex non-polar SASA 627.1Ų
Non-polar SASA of full complex (VdW proxy, nonpolar atoms only)