FAIRMol

Z20229393

Pose ID 480 Compound 4 Pose 480

DB SELECTIONDocking pose analysis is being read from this database.
Molecular metrics status: done
RDKit SASA-based burial metrics are cached.
SASA cached
T01
Human DHFR Human
Ligand Z20229393
PDB5SD8

3D complex viewer

Strict H-bonds Permissive H-bonds
Viewer legend
Protein receptor
Pocket residues
Cofactor context
Docked ligand
Overall assessment
Promising SASA cached
Promising and worth follow-up
Binding strong Geometry high Native mixed SASA done
Strain ΔE
13.2 kcal/mol
Protein clashes
1
Internal clashes
1
Native overlap
contact recall 0.52, Jaccard 0.44, H-bond role recall 0.00
Burial
85%
Hydrophobic fit
67%
Reason: no major geometry red flags detected
1 protein-contact clashes
Molecular report
Full metrics ↗
Promising Reasonable quality metrics. Warrants further investigation.
✓ Excellent LE (-1.052 kcal/mol/HA) ✓ Good fit quality (FQ -9.43) ✓ Deep burial (85% SASA buried) ✓ Lipophilic contacts well-matched (67%) ✗ Moderate strain (13.2 kcal/mol) ✗ Geometry warnings ✗ Minor protein-contact clashes (2) ✗ Internal clashes (6)
Score
-25.241
kcal/mol
LE
-1.052
kcal/mol/HA
Fit Quality
-9.43
FQ (Leeson)
HAC
24
heavy atoms
MW
354
Da
LogP
4.65
cLogP
Strain ΔE
13.2 kcal/mol
SASA buried
85%
Lipo contact
67% BSA apolar/total
SASA unbound
592 Ų
Apolar buried
337 Ų

Interaction summary

HB 2 HY 24 PI 3 CLASH 1
Final rank0.045Score-25.241
Inter norm-1.090Intra norm0.039
Top1000noExcludedno
Contacts15H-bonds2
Artifact reasongeometry warning; 6 clashes; 2 protein contact clashes
Residues
ALA10 ARG29 GLU31 ILE8 LEU23 LEU28 NAP201 PHE32 PHE35 PRO27 THR137 TRP25 TYR122 VAL116 VAL9

Protein summary

200 residues
Protein targetT01Atoms3128
Residues200Chains2
Residue summaryLYS:374; LEU:361; GLU:240; VAL:224; ARG:192; PHE:180; ILE:171; PRO:168; ASN:140; SER:132; TYR:126; GLN:119; ASP:108; MET:102; THR:98; GLY:91

Receptor context

1 kept / 0 excluded
Receptor context filtering: interactions and SASA are computed against protein atoms plus allowed cofactors/ions. Native ligand-like HETATM partners are excluded from scoring.
Kept context 1 Excluded HETATM 0
Kept cofactors / ions
A:NAP201

Native ligand reference

★ reference
Interaction fingerprint calculated directly from the uploaded native ligand without docking. Current H-bond mode: strict.
Name5SD8Contacts21
PoseOpen native poseHB0
IFP residues
ALA10 ASN65 ASP22 GLN36 GLU31 GLY117 GLY21 ILE61 ILE8 LEU23 LEU68 NAP201 PHE32 PHE35 PRO62 SER60 THR137 THR57 TYR122 VAL116 VAL9
Current overlap11Native recall0.52
Jaccard0.44RMSD-
HB strict0Strict recall0.00
HB same residue+role0HB role recall0.00
HB same residue0HB residue recall0.00

HB · H-bonds

Mode: strict. Count shows atom-level H-bonds; unique residues in summary: 0.

PI · π–π interactions

Native π–π recall is disabled because no explicit native π–π reference was stored.

HY · Hydrophobic contacts

CLASH · Clashes

All stored poses for this docking hit

PoseFinal rankInter normScoreHBCTCT overlapCT recallHB role rec.RMSDExcluded
389 -0.18784752275886354 -1.01592 -23.8653 1 17 0 0.00 0.00 - no Open
480 0.044810234807672045 -1.09043 -25.2406 2 15 11 0.52 0.00 - no Current
471 0.05669390183562073 -1.09591 -25.194 3 16 12 0.57 0.00 - no Open
419 0.2488068813182308 -1.18196 -27.8753 4 15 0 0.00 0.00 - no Open
441 1.2946467321758754 -1.18634 -27.2383 3 13 0 0.00 0.00 - no Open
383 2.0117884778778174 -1.01577 -22.8536 10 15 0 0.00 0.00 - no Open
372 2.25182953308986 -1.35937 -32.3799 4 11 0 0.00 0.00 - no Open
390 2.8542622317418695 -1.17081 -26.8659 9 15 0 0.00 0.00 - no Open

Molecular metrics

RDKit SASA burial, strain energy (MMFF94s), ligand efficiency and fit quality for this docking pose.
✓ Metrics available

Scoring & efficiency

Docking score -25.241kcal/mol
Ligand efficiency (LE) -1.0517kcal/mol/HA
Score / heavy atom count
Fit quality (FQ) -9.425
LE / (0.072 + 0.95/HAC) — Leeson & Springthorpe
Heavy atom count 24HA

Physicochemical properties

Molecular weight 354.5Da
Lipinski: ≤ 500 Da
LogP (cLogP) 4.65
Lipinski: ≤ 5
Rotatable bonds 4

Conformational strain (MMFF94s)

Strain energy (ΔE) 13.22kcal/mol
< 5 good · 5–10 marginal · > 10 problematic
Docked FF energy 17.25kcal/mol
Minimised FF energy 4.03kcal/mol

SASA & burial

✓ computed
SASA (unbound) 592.1Ų
Total solvent-accessible surface area of free ligand
BSA total 503.8Ų
Buried surface area upon binding
BSA apolar 337.5Ų
Hydrophobic contacts buried
BSA polar 166.3Ų
Polar contacts buried
Fraction buried 85.1%
> 60 % indicates good pocket engagement
Lipophilic contact ratio 67.0%
BSA apolar / BSA total — high = hydrophobic driver
Δ Non-polar SASA -1497.2Ų
SASA_nonpolar(complex) − SASA_nonpolar(receptor) − SASA_nonpolar(ligand free). Negative = non-polar surface buried upon binding. Requires full polarity-decomposed SASA computation.
Receptor non-polar SASA 1677.9Ų
Non-polar SASA of receptor alone (VdW proxy, nonpolar atoms only)
Complex non-polar SASA 606.5Ų
Non-polar SASA of full complex (VdW proxy, nonpolar atoms only)